Reproduction Abstracts

Somatic cell nuclear transfer (SCNT) has a broad spectrum of many potential applications but the efficiency is still disappointingly low. It is known that major cause of it, is incorrect or incomplete reprogramming of the transferred donor nuclei.Many report indicates that Wnt/β-catenin signaling pathway plays a pivotal role in the process of somatic cell reprogramming. As CHIR is known as one of the activators of Wnt pathway by inhibiting glycogen ...

Introduction: The type II bacterial clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein (Cas) have been proven to be an effective gene targeting system. Genome editing of non-rodent mammalian species is a promising strategy for generation of animal models for human diseases. Mashiko et al. reported a high efficacy of direct injection of a plasmid DNA, encoding humanized Cas9 and sgRNA (single-guide RNA) in mice. Here we show successf...

Introduction: Trophoblast stem cells (TSC) maintain their undifferentiated status under the presence of fibroblast growth factor 4 (FGF4), heparin and feeder cell-conditioned medium (CM). It has been reported that activin A can replace CM. However, even in such stem cell condition, a portion of TSC spontaneously undergoes differentiation. In addition, when TSC are induced to differentiate, all trophoblast subtypes autonomously appear without the addition of any exogenous facto...